Human MCP-1/MCAF ELISA KIT (CAT#: VCye-Wyb011)

• Product Overview
• Human MCP-1/MCAF ELISA KIT is used for quantitative determination of human monocyte chemotactic protein 1/monocyte chemotactic and activating factor (MCP-1/MCAF) concentrations in serum, plasma, urine, saliva, cerebrospinal fluid (CSF), ascitic fluid, cell culture supernates, tissue homogenates.

• Sensitivity
• 30.184 pg/mL

• Target
• SARS-CoV-2

• Speicies
• Human

• Contents
• Assay plate (12 x 8 coated Microwells), Standard, Biotin-antibody, HRP-avidin, Biotin-antibody Diluent, HRP-avidin Diluent, Sample Diluent, Wash Buffer (25 x concentrate), TMB Substrate, Stop Solution, Adhesive Strip (For 96 wells).

• Testing Principle
• Quantitative ELISA

• Detection Wavelength
• 450 nm

• Detection Range
• 31.25 pg/mL-2000 pg/mL

• Precision
• Intra-assay Precision: CV% < 8%
  Inter-assay Precision: CV% < 10%

• Sample Types
• Serum, plasma, urine, saliva, cerebrospinal fluid (CSF), ascitic fluid, cell culture supernates, tissue homogenates

• Sample Volume
• 50-100 μL

• Storage
• Store at 2-8°C.

• Introduction
• Monocyte chemoattractant protein 1 (MCP-1) belongs to a small cytokine of the CC chemokine family. Studies have shown that MCP-1 is an important proinflammatory cytokine. Monocytes, macrophages, fibroblasts, vascular endothelial cells, B cells, smooth muscle cells, etc. can all secret MCP-1 when inflammation occurs in the body. and MCP-1 has a specific chemotactic activation effect on monocytes / macrophages. In particular, it plays an important role in the occurrence and development of rheumatoid arthritis, glomerulonephritis, atherosclerosis, acute coronary syndrome, deep vein thrombosis and other diseases.

• Alternative Names
• CCL2, chemokine (C-C motif) ligand 2, C-C motif chemokine 2, GDCF 2, GDCF2, MCP-1, MCAF, Human monocyte chemotactic protein 1, monocyte chemotactic and activating factor