SARS-CoV-2 CPE Assay Service

SARS-CoV-2, a novel coronavirus strain, has spread to almost every country in the world and thus become a major public health concern. Researchers all over the world are trying their best to improve the current situation in different methods. Cytopathic Effect (CPE) assay is one of the antiviral tests that may help with discovering more information about SARS-CoV-2. As a socially responsible company focused on antiviral research, Creative Biolabs has been committed to SARS-CoV-2 and relative studies since it broke out. With advanced technology and mature researchers, we provide global clients with reliable CPE assay services to promote the study of SARS-CoV-2.

SARS-CoV-2 CPE Assay Service

Introduction of CPE

Various pathological cell alterations triggered by eukaryotic viruses are referred to as the CPE. Due to viral infections, the structure of the host cell changes and sometimes results in cell lysis. Common CPE includes rounding, detachment (plaques), clumping, ballooning (giant cell), fusion (syncytium formation), and inclusion body formation. The formation of inclusion bodies or syncytia induced by CPE can help with diagnosing viral infections. Therefore, by CPE, viral proliferation could be estimated through observable changes in cultured cells under a light microscope. In summary, CPE is a feasible scheme for viral isolation and identification.

Not only in the drug antiviral effect investigation, CPE assay was reported to be applied but also in methodology research of viral growth, titration, and neutralization of SARS-CoV-2 presented that is capable of the quantification of the neutralizing antibody titer in serum samples.

Reduction of SARS-CoV-2-induced cytopathic effect by LS.Fig.1 Reduction of SARS-CoV-2-induced cytopathic effect by LS. (Ma, 2020)

Services at Creative Biolabs

Committed to antiviral drug discovery and vaccine development for years, Creative Biolabs has accumulated extensive professional experience from hundreds of customer-satisfied projects. With advanced technologies, mature technician, and excellent experts, we have established a reliable platform to provide high-quality antiviral drug discovery services for SARS-CoV-2 to global customers.

Workflow for CPE Assay of SARS-CoV-2 at Creative BiolabsFig.2 Workflow for CPE Assay of SARS-CoV-2 at Creative Biolabs

Effector Cell Vero E6 cells
Detection Scheme
  • Prepare 96-well plates of the desired cell line and incubate overnight;
  • 8 half-log serial dilutions in duplicate in the test medium;
  • Infected with the test virus diluted in the test medium;
  • Incubate at 37°C, 5% CO2 until CPE is apparent;
  • Stain with neutral red dye for 2 hours;
  • Add 200 µL 50:50 Sorensen citrate buffer/ethanol for >30 min., agitate, and read on a spectrophotometer at 540 nm;
  • Calculate EC50 and CC50 by regression analysis.
Positive Control
  • Remdesivir
  • Other compounds provided by the client
Requirements for Compounds Powders or pre-solubilized DMSO stock
Deliverables EC50; CC50

Why Us?

  • Professional experts with Ph.D. level degrees
  • Hundreds of customer-satisfied precedents
  • Effective services with competitive price
  • Great industry reputation

The spread of SARS-CoV-2 is a difficult period we need to face together. As an industry-leading company, Creative Biolabs is duty-bound to be on the front line of the fright against SARS-CoV-2 and has done so. With a comprehensive platform and specialists with advanced degrees, we offer reliable SARS-CoV-2 the CPE assay services to facilitate global customers. If you are interested in CPE assay, please feel free to contact us for more information.

Reference

  1. Ma, Q.; et al. Liu Shen capsule shows antiviral and anti-inflammatory abilities against novel coronavirus SARS-CoV-2 via suppression of NF-κB signaling pathway. Pharmacol Res. 2020, 158: 104850.
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