rRT-PCR for the Detection of SARS-CoV-2

Creative Biolabs is a leading service provider that focuses on SARS-CoV-2 drug discovery services. Currently, various platforms have been established for screening SARS-CoV-2 in different population. We have successfully established a series of Real-time Reverse Transcription PCR (rRT-PCR) assays for identifying SARS-CoV-2 and developing a series of IVD kits for our worldwide clients.

Introduction to rRT-PCR

A novel coronavirus (SARS-CoV-2) has been shown to be highly contagious and to cause many severe respiratory diseases in humans. The virus is a type of coronaviruses that has a single-stranded RNA genome, which is responsible for encoding several key proteins. With the outbreak of SARS-CoV-2 in Wuhan, Hubei Province, China, a number of in-vitro diagnostics (IVD) kits have been developed by using various PCR-based technologies, such as rRT-PCR, for screening the virus emergence in different populations. rRT-PCR has been considered as a benchmark for detecting and quantifying RNA targets and is being broadly used in clinical diagnosis. In rRT-PCR assays, an oligonucleotide probe will be designed to bind with the key sequence of SARS-CoV-2. Meanwhile, the probe will cleave when the Taq polymerase is added into this detection system. The cleavage will be detected once the fluorescent signal being released.

Fig.1 The principle of the 5’ nuclease (TaqMan) assay. (Stephen, 2005)

Our Services for rRT-PCR

In the past few years, rRT-PCR has become the standard assay for screening RNA targets in different coronavirus subtypes, including MERS-CoV and SARS-CoV. As an expert in drug discovery, Creative Biolabs offers a full range of IVD kits development services for our clients to help identify SARS-CoV-2 in a fast and cost-effective manner. Our rRT-PCR assays are high-throughput detection tools with enhanced chemistries, more suitable PCR systems, as well as strict protocols. Among them, the TaqMan assay has become a popular way for monitoring the PCR reaction system and detecting quantifying SARS-CoV-2 mRNA. In particular, this assay has many advantages for detecting SARS-CoV-2, including but not limited to：

• Using fluorescent reporter to monitor each cycle of PCR amplification
• Allowing for analyzing samples with different target abundance
• Realizing the inherent quantitative ability of PCR assay
• More stable PCR assays that can easily obtain reproducible results

With the help of abundant experience and professional scientists, Creative Biolabs is committed to guiding global customers to a range of SARS-CoV-2 drug discovery services. Our laboratory services have many advanced chemical and biological technologies to support the requirement of our customers in the fast-growing SARS-CoV-2 research. If you are interested in our services, please feel free to contact us or send us an inquiry.

Reference

1. Stephen, A. B.; et al. Real-time reverse transcription PCR (qRT-PCR) and its potential use in clinical diagnosis. Clinical Science. 2005, 109: 365-379.

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