Reverse Genetics Systems Targeting SARS-CoV-2

Creative Biolabs has an entrepreneurial team of highly-skilled and qualified experts in drug screening and drug development. The members have originated from a rich academic research background, bringing their experience into translation. We are very proud of providing high-quality, omnidirectional reverse genetics systems services targeting SARS-CoV-2 to our valued clients to remove the difficulties of your projects.

Introduction to Reverse Genetics Systems

Currently, a novel coronavirus (SARS-CoV-2) has been identified in many countries and it has become a critical issue to global public health due to high morbidity in human populations. Meanwhile, pilot studies have shown that reverse genetics systems play an important role in studying the source, the replication cycle, and the pathophysiology of RNA viruses, such as the Zika virus (ZIKV) and coronavirus, as well as developing novel antivirals or therapies for clinical use. In general, reverse genetics systems are generated based on infectious clone technology. The intron sequences encoding various proteins and a luciferase reporter gene will be broadly used to improve the stability of these systems. Moreover, the infectious subgenomic-amplicons (ISA) method has also been established to express wild-type and recombinant coronavirus with the GFP reporter. The results collected from these assays have suggested that reverse genetics systems are powerful tools for discovering new drugs for treating virus infection. Therefore, reverse genetics systems should be a potential strategy for detecting the physiopathology of SARS-CoV-2 in preclinical studies.

Systematic assembly of a SARS-CoV full-length cDNA clone by <em>in vitro</em> ligation. Fig.1 Systematic assembly of a SARS-CoV full-length cDNA clone by in vitro ligation. (Almazán, 2014)

Our Reverse Genetics Systems Targeting SARS-CoV-2

SARS-CoV-2, a typical example of coronavirus, recently caused large outbreaks in a number of countries, associated with respiratory diseases in different populations. Reverse genetics systems are capable of generating specific viruses, including coronavirus, which have been applied in target screening and drug discovery. Furthermore, reverse genetics systems are critical to studying the lifecycle of different viruses without a large scale containment laboratory.

With the commitment of being your best antiviral drug discovery partner, Creative Biolabs has established the utmost efficient integrated solutions to innovate and accelerate your development process for various diseases. Equipped with a team of professional scientists, we have generated a number of reverse genetics systems for our clients to study life cycle, replication, transcription, and pathophysiology of SARS-CoV-2. Targeted RNA recombination of SARS-CoV-2 can be a multifunctional, powerful tool for analyzing the SARS-CoV-2 genome and genetic mutations in different evolutionary time-scales.

With extensive expertise and experience in the field of antiviral drug development, Creative Biolabs has established many reverse genetics systems for offering SARS-CoV-2 targeted preclinical studies tailored to your specifications. If you are interested in our services, please contact us for more details. Let us know what you need and we will accommodate you. We look forward to working with you in the future.

Reference

  1. Almazán, F.; et al. Coronavirus reverse genetic systems: infectious clones and replicons. Virus Res. 2014, 189: 262-70.
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