Human TNF-α ELISA KIT (CAT#: VCye-Wyb013)

• Product Overview
• Human TNF-α ELISA KIT is used for quantitative determination of human tumor necrosis factor α (TNF-α) concentrations in serum, plasma, cell culture supernates, tissue homogenates, cell lysates.

• Sensitivity
• 1.95 pg/mL

• Target
• SARS-CoV-2

• Speicies
• Human

• Contents
• Assay plate (12 x 8 coated Microwells), Standard, Biotin-antibody, HRP-avidin, Biotin-antibody Diluent, HRP-avidin Diluent, Sample Diluent, Wash Buffer (25 x concentrate), TMB Substrate, Stop Solution, Adhesive Strip (For 96 wells).

• Testing Principle
• Quantitative ELISA

• Detection Wavelength
• 450 nm

• Detection Range
• 7.8 pg/mL-500 pg/mL

• Precision
• Intra-assay Precision: CV% < 8%
  Inter-assay Precision: CV% < 10%

• Average Recovery
• 0.96

• Sample Types
• Serum, plasma, cell culture supernates, tissue homogenates, cell lysates

• Sample Volume
• 50-100 μL

• Storage
• Store at 2-8°C.

• Introduction
• Tumor necrosis factor (TNF) is the earliest discovered cytokine that can cause hemorrhagic necrosis of tumor tissue cells, mainly secreted by macrophages and fat cells in adipose tissue. It is named because it can directly kill tumor cells in vivo and in vitro. According to their source and structure, they are mainly divided into TNF-α and TNF-β. TNF-α is mainly produced by activated mononuclear-macrophages and various other cells. Large doses of TNF-α can cause cachexia, and patients show progressive weight loss, so TNF-α is also known as cachexia.

• Alternative Names
• TNF-α, Human Tumor necrosis factor α, tumor necrosis factor, APC1, Differentiation inducing factor, Macrophage cytotoxic factor