Human IFN-γ ELISA Kit (CAT#: VCye-Wyb001)

• Product Overview
• Human IFN-γ ELISA Kit is used for quantitative determination of human interferon γ (IFN-γ) concentrations in serum, cell culture supernates, urine, tissue homogenates and cerebrospinal fluid (CSF).

• Sensitivity
• 1.56 pg/mL

• Target
• SARS-CoV-2

• Speicies
• Human

• Contents
• Assay plate (12 x 8 coated Microwells), Standard, Biotin-antibody, HRP-avidin, Biotin-antibody Diluent, HRP-avidin Diluent, Sample Diluent, Wash Buffer (25 x concentrate), TMB Substrate, Stop Solution, Adhesive Strip (For 96 wells).

• Testing Principle
• Quantitative ELISA

• Detection Wavelength
• 450 nm

• Detection Range
• 6.25 pg/mL-400 pg/Ml

• Precision
• Intra-assay Precision: CV% < 8%
  Inter-assay Precision: CV% < 10%

• Average Recovery
• 0.94

• Sample Types
• Serum, cell culture supernates, urine, tissue homogenates, cerebrospinal fluid.

• Sample Volume
• 50-100 μL

• Storage
• Store at 2-8°C.

• Introduction
• Interferon is the earliest discovered cytokine. It is a low-molecular-weight multifunctional glycoprotein produced by viruses and interferon inducers acting on host cells. It is named for its ability to interfere with viral infection and replication. Interferon can be divided into type Ⅰ and type Ⅱ interferon. Type Ⅰ interferon includes IFN-α, IFN-β, IFN-κ, etc. IFN-γ belongs to type Ⅱ interferon, produced by activated T cells and NK cells. It can activate macrophages, promote the expression of MHC molecules, induce Th1 cell differentiation, and inhibit Th2 cell differentiation.

• Alternative Names
• IFNG, IFN-γ, IFN gamma, Type II Interferon, Interferon, Interferon γ