Human IL-1β ELISA KIT (CAT#: VCye-Wyb006)

• Product Overview
• Human IL-1β ELISA KIT is used for quantitative determination of human interleukin 1β (IL-1β) concentrations in serum, cell culture supernates, urine, cerebrospinal fluid (CSF).

• Sensitivity
• 31.25 pg/mL

• Target
• SARS-CoV-2

• Speicies
• Human

• Contents
• Assay plate (12 x 8 coated Microwells), Standard, Biotin-antibody, HRP-avidin, Biotin-antibody Diluent, HRP-avidin Diluent, Sample Diluent, Wash Buffer (25 x concentrate), TMB Substrate, Stop Solution, Adhesive Strip (For 96 wells).

• Testing Principle
• Quantitative ELISA

• Detection Wavelength
• 450 nm

• Detection Range
• 125 pg/mL-8000 pg/mL

• Precision
• Intra-assay Precision: CV% < 8%
  Inter-assay Precision: CV% < 10%

• Average Recovery
• 0.87

• Sample Types
• Serum, cell culture supernates, urine, cerebrospinal fluid (CSF).

• Sample Volume
• 50-100 μL

• Storage
• Store at 2-8°C.

• Introduction
• IL-1β is also known as catabolin, and its precursor is cleaved by caspase 1. Cytosolic thiol protease cleaves the product to form mature IL-1β. IL1β are structurally related polypeptides that share approximately 21% aa identity in human. Both proteins are produced by a wide variety of cells in response to inflammatory agents, infections, or microbial endotoxins. IL1α and IL1β bind to the same receptor and exert identical biological effects. IL-1β is produced by activated macrophages as a proprotein, which is proteolytically processed to its active form by caspase 1 (CASP1/ICE). This cytokine is an important mediator of the inflammatory response, and is involved in a variety of cellular activities, including cell proliferation, differentiation, and apoptosis.

• Alternative Names
• IL-1β, Interleukin 1β, Catabolin, IFN beta inducing factor