Human IL-1β ELISA KIT (CAT#: VCye-Wyb006)

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For Research Use Only. We do not provide direct services or products for patients.

  • Product Overview
  • Human IL-1β ELISA KIT is used for quantitative determination of human interleukin 1β (IL-1β) concentrations in serum, cell culture supernates, urine, cerebrospinal fluid (CSF).
  • Sensitivity
  • 31.25 pg/mL
  • Target
  • SARS-CoV-2
  • Speicies
  • Human
  • Contents
  • Assay plate (12 x 8 coated Microwells), Standard, Biotin-antibody, HRP-avidin, Biotin-antibody Diluent, HRP-avidin Diluent, Sample Diluent, Wash Buffer (25 x concentrate), TMB Substrate, Stop Solution, Adhesive Strip (For 96 wells).
  • Testing Principle
  • Quantitative ELISA
  • Detection Wavelength
  • 450 nm
  • Detection Range
  • 125 pg/mL-8000 pg/mL
  • Precision
  • Intra-assay Precision: CV% < 8%
    Inter-assay Precision: CV% < 10%
  • Average Recovery
  • 0.87
  • Sample Types
  • Serum, cell culture supernates, urine, cerebrospinal fluid (CSF).
  • Sample Volume
  • 50-100 μL
  • Storage
  • Store at 2-8°C.
  • Introduction
  • IL-1β is also known as catabolin, and its precursor is cleaved by caspase 1. Cytosolic thiol protease cleaves the product to form mature IL-1β. IL1β are structurally related polypeptides that share approximately 21% aa identity in human. Both proteins are produced by a wide variety of cells in response to inflammatory agents, infections, or microbial endotoxins. IL1α and IL1β bind to the same receptor and exert identical biological effects. IL-1β is produced by activated macrophages as a proprotein, which is proteolytically processed to its active form by caspase 1 (CASP1/ICE). This cytokine is an important mediator of the inflammatory response, and is involved in a variety of cellular activities, including cell proliferation, differentiation, and apoptosis.
  • Alternative Names
  • IL-1β, Interleukin 1β, Catabolin, IFN beta inducing factor

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