SARS-CoV Spike-pseudovirus was prepared by co-transfecting 293T cells with an HIV-1 backbone plasmid and a plasmid containing the SARS-CoV spike encoding gene. The packaged Spike-pseudovirus expresses full-length spike protein on the surface and carries a GFP reporter gene, which can be used to infect cells that overexpress ACE2, and express GFP in the cells to characterize infectivity of the virus.
Product Type
Pseudovirus
Target
SARS-CoV
Contents
SARS-CoV Spike full-length gene.
Backbone
Lentiviral vector
Reporter Gene
GFP
Variant
WT
Applications
Neutralizing antibody / serum test / As a standard for ELISA and other tests.
Storage
Store at -20°C to -80°C
Introduction
Severe acute respiratory syndrome coronavirus 1 (SARS-CoV) is a strain of coronavirus that causes severe acute respiratory syndrome (SARS), the respiratory illness responsible for the 2002-2004 SARS outbreak. It is an enveloped, positive-sense, single-stranded RNA virus that infects the epithelial cells within the lungs. The virus enters the host cell by binding to angiotensin-converting enzyme 2. A virus very similar to SARS was discovered in late 2019. This virus, named severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), is the causative pathogen of COVID-19, the propagation of which started the COVID-19 pandemic.