SARS-CoV Spike-pseudovirus was prepared by co-transfecting 293T cells with an HIV-1 backbone plasmid and a plasmid containing the SARS-CoV spike encoding gene. The packaged Spike-pseudovirus expresses full-length spike protein on the surface and carries a luciferase and GFP reporter gene, which can be used to infect cells that overexpress ACE2, and express luciferase and GFP in the cells to characterize infectivity of the virus.
Product Type
Pseudovirus
Target
SARS-CoV
Contents
SARS-CoV Spike full-length gene.
Backbone
Lentiviral vector
Reporter Gene
Luc/GFP
Variant
WT
Applications
Neutralizing antibody / serum test / As a standard for ELISA and other tests.
Storage
Store at -20°C to -80°C
Introduction
Severe acute respiratory syndrome coronavirus 1 (SARS-CoV) is a strain of coronavirus that causes severe acute respiratory syndrome (SARS), the respiratory illness responsible for the 2002-2004 SARS outbreak. It is an enveloped, positive-sense, single-stranded RNA virus that infects the epithelial cells within the lungs. The virus enters the host cell by binding to angiotensin-converting enzyme 2. A virus very similar to SARS was discovered in late 2019. This virus, named severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), is the causative pathogen of COVID-19, the propagation of which started the COVID-19 pandemic.