Remdesivir is a cyano-substituted adenosine nucleotide analog prodrug and shows broad-spectrum antiviral activity against several RNA viruses. In HAE cells, Remdesivir has an EC50 value of 74 nM for SARS-CoV and MERS-CoV, and 30 nM for murine hepatitis virus in delayed brain tumor cells. In vitro experiments and animal model data indicate that remdesivir inhibits SARS-CoV, MERS-CoV, and Ebola virus by inhibiting RNA-dependent RNA polymerase (RdRp). In vitro cell experiments showed that the half effective concentration (EC50) of remdesivir for the new coronavirus was 0.77 μmol·L-1.