Peptide Microarray Analysis for SARS-CoV-2

In December 2019, pneumonia of unknown cause occurred in Wuhan, China. A previously unknown beta-coronavirus was discovered through the use of unbiased sequencing in samples from patients with pneumonia. After a series of sequencing and analyzing the samples, this unknown coronavirus is  proved a coronavirus, named SARS-CoV-2. Creative Biolabs is committed to providing high-quality biological products and customized solutions to advance our global customer’ research projects. Due to the serious condition in the world, especially China, we offer peptide microarray analysis services for SARS-CoV-2 to help scientists to research the drug discovery and development of SARS-CoV-2.

Introduction

Different from the severe acute respiratory syndrome coronavirus (SARS-CoV) and Middle East respiratory syndrome coronavirus (MERS-CoV) in the past, SARS-CoV-2 is the seventh member of the family of coronaviruses that can infect humans. In order to support the researches and treatment of SARS-CoV-2, Creative Biolabs now provides the peptide microarray analysis as the powerful tool for dissecting and researching the drugs or antibodies or/and inhibitors of SARS-CoV-2. This analysis service can profile the fine specificity of antibody binding against thousands of peptides simultaneously. Moreover, peptide microarray analysis is more stable than proteins on microarray which is useful in the design of peptide-based inhibitors or drugs that can be used to modulate protein activity. Therefore, Creative Biolabs offers the peptide microarray analysis for the drug discovery and development of SARS-CoV-2.

Principle of Peptide Microarray Analysis

A peptide microarray, also called peptide chip or peptide epitope microarray, is a tool that is able to collect peptides and then display the peptides on a solid surface, usually a glass or plastic chip. The assay principle of peptide microarrays is similar to an ELISA protocol, shown in Fig.1. The peptides (up to tens of thousands in several copies) are linked to the surface of a glass chip typically the size and shape of a microscope slide. This chip can directly be incubated with a range of different biological samples like purified enzymes or antibodies, patient or animal sera, cell lysates and then be detected through a label-dependent fashion. Through these processes, scientists can find the desired target candidates.

Fig.1 The principle of peptide microarray analysis.

Applications of Peptide Microarrays

Peptide microarrays, in particular, are crucial tools for high-throughput screening. Peptide microarrays can be used to:

• Epitope-mapping
• Substrate profiling
• Probing peptide-ligand interactions
• Protein interaction domains

Besides that, peptide microarrays are utilized by scientists in biology, medicine and pharmacology to research binding properties and functionality and kinetics of protein-protein interactions in general. Within the basic research, peptide microarrays are commonly used to profile an enzyme (like kinase, phosphatase, protease, acetyltransferase, histone deacetylase and so on.) to map an antibody epitope or find out the key residues for protein binding. All applications are useful for the drug discovery and development of SARS-CoV-2.

Advantages of Peptide Microarray Analysis

• Easy to operate
• Low cost of synthesis
• More stable
• Detection of binding events on the epitope level
• Flexible design for peptide sequence based on clients’ requirements
• Higher batch-to-batch reproducibility

Based on advanced technologies and experienced researchers, Creative Biolabs is dedicated to providing high-class peptide microarray analysis service to support the drug discovery and development of SARS-CoV-2. If you are interested in our peptide microarray analysis service technologies, please feel free to contact us.

For Research Use Only. We do not provide direct services or products for patients.