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The N gene sequence of the SARS-CoV-2 (2019-nCoV, reference sequence NC_045512) was synthesized and cloned into a lentiviral vector, and the pseudovirus was prepared in 293T cells. The obtained pseudovirus has an RNA sequence containing the N gene 1260 base in the lentiviral genome. It can be used as a reference for viral RNA extraction experiments and qPCR detection experiments.
Contains an RNA sequence containing the N gene 1260 base in the lentiviral genome
108 virus copies/mL
Used as a reference for viral RNA extraction experiments and qPCR detection experiments.
Store at -80°C.
The 2019 novel coronavirus, or "SARS-CoV-2", was discovered because of Wuhan virus pneumonia cases in 2019, and was named by the World Health Organization on January 12, 2020. It belongs to the beta genera of the Coronaviridae family, together with SARS coronavirus in 2003 and MERS coronavirus in 2012. The alignment between SARS-CoV-2 and 2003 SARS CoV has about 70% sequence similarity and 40% sequence similarity with MERS CoV. The coronavirus genome encodes a spike protein, an envelope protein, a membrane protein, and a nucleoprotein. Among them, spike protein is the most important surface membrane protein of coronavirus.