For Research Use Only. We do not provide direct services or products for patients.
Product Overview
The SARS-CoV-2 (2019-nCoV) ORF1ab qRT-PCR (Taqman™) Detection Kit is designed based on the gene sequences of ORF1ab gene of SARS-CoV-2 genome and is used for the quantitative detection of ORF1ab gene. The kit is for research use only, not for diagnoses.
Target
SARS-CoV-2
Specificity
ORF1ab gene of SARS-CoV-2 virus genome.
Contents
One step mix; one step enzyme mix; 50×ROX dye 1; 50×ROX dye 2; forward primer; reverse primer; probe; RNase-free water; positive control plasmid (1 pg/μl).
Testing
SARS-CoV-2 ORF1ab
Testing Principle
qRT-PCR (Taqman™)
Applications
Quantitative measurement of SARS-CoV-2 (2019-nCoV) ORF1ab gene.
Storage
Store at -20 ± 5°C
Introduction
The 2019 novel coronavirus, or "SARS-CoV-2", was discovered because of Wuhan virus pneumonia cases in 2019, and was named by the World Health Organization on January 12, 2020. It belongs to the beta genera of the Coronaviridae family, together with SARS coronavirus in 2003 and MERS coronavirus in 2012. The alignment between SARS-CoV-2 and 2003 SARS CoV has about 70% sequence similarity and 40% sequence similarity with MERS CoV. The coronavirus genome encodes a spike protein, an envelope protein, a membrane protein, and a nucleoprotein. Among them, spike protein is the most important surface membrane protein of coronavirus.