SARS-CoV-2 (2019-nCoV) One-Step RT-PCR Mix includes the latest advances in buffer chemistry and PCR enhancers and stabilizers, with an antibody-mediated hot-start polymerase, dNTPs and MgCl2 and separate reverse transcriptase and Rnase inhibitor.
Target
SARS-CoV-2
Contents
One-Step RT-PCR Mix; MMLV-RT; RNase inhibitor.
Testing
SARS-CoV-2
Testing Principle
Real-time RT-PCR
Applications
RT-PCR
Storage
Store at -20°C
Introduction
The 2019 novel coronavirus, or "SARS-CoV-2", was discovered because of Wuhan virus pneumonia cases in 2019, and was named by the World Health Organization on January 12, 2020. It belongs to the beta genera of the Coronaviridae family, together with SARS coronavirus in 2003 and MERS coronavirus in 2012. The alignment between SARS-CoV-2 and 2003 SARS CoV has about 70% sequence similarity and 40% sequence similarity with MERS CoV. The coronavirus genome encodes a spike protein, an envelope protein, a membrane protein, and a nucleoprotein. Among them, spike protein is the most important surface membrane protein of coronavirus.